Part:BBa_K3332080

pBAD/araC promoter-cI repressor-terminator

It can express cI repressor under certain conditions.By using this part, we can make engineering bacteria survive at the early growth stage，which is very sensitive to formaldehyde.

Usage and Biology

We want to use this part with BBa_K3332081. The promoter pBAD/araC is induced under arabinose and cI repressor is the co-protein of MazF, reduces the toxicity of MazF after their combination, provides the minimum margin of formaldehyde-induced cell toxicity.

Fig 1. pBAD _B0034_cI_B0015

Characterization

When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify the ligation. After the circuit was built, we sent the plasmid to sequence, and got the correct result. After new molecular cloning experiments, we did Enzyme-Cut identification for further confirmation. We used the EcoR I and Pst I to cut the plasmid, then we got the target separate fragment.

Fig 2.I0500_B0034_cI_B0015_pSB1C3[BBa_K3332080] digested by EcoR I and Pst I

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961